ALKALINE PHOSPHATASE ACTIVITY IN HELA CELLS

The induction of alkaline phosphatase activity in HeLa cells by 5-iodo-2'-deoxyuridine.

uridine (lUdR) there was an increase in alkaline phosphatase activity after about 24 hr, reaching a maximum usually at about 72 hi. At this concentration, during this time, there was a relatively small inhibition of cell replication. The increment in alkaline phosphatase activity was in large measure prevented by concomitant addition of 10 /¿Mthymidine. Further, lUdR did not increase alkaline ...

Induction of alkaline phosphatase activity in HeLa cells by choline chloride.

Choline chloride produced a dose-dependent induction of alkaline phosphatase activity in HeLa cells. At the highest concentration tested, 40 mM, there was a 5- to 7-fold increase in alkaline phosphatase activity, a significantly greater induction than that produced by equiosmolar additions of either NaCl or sucrose. Enzyme activity was higher than control values by 24 hr after the addition of t...

Alkaline Phosphatase in Stem Cells

Alkaline phosphatase is an enzyme commonly expressed in almost all living organisms. In humans and other mammals, determinations of the expression and activity of alkaline phosphatase have frequently been used for cell determination in developmental studies and/or within clinical trials. Alkaline phosphatase also seems to be one of the key markers in the identification of pluripotent embryonic ...

Cell Cycle Events in the Hydrocortisone Regulation of Alkaline Phosphatase in Hela S3 Cells

The increase in alkaline phosphatase in asynchronous cultures of HeLa S(3) cells grown in medium supplemented with hydrocortisone is characterized by a lag period of 10-12 hr. Present studies utilizing synchronous cell populations indicate: (a) a minimum of 8-10 hr of incubation with hydrocortisone is necessary for maximum induction of alkaline phosphatase; (b) the increase in enzyme activity p...

Skeletal alkaline phosphatase activity in serum.